Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]

CAT#: AM20255FC-N

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), FITC

Specifications

Product Data

• Clone Name: NI 330 (HP 6050)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect Immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
  General Recommended Dilutions:
  Histochemical Use: 1/10-1/50
  ELISA: from 1/200 upwards
  Western blot: from 1/400 upwards
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgG3 isolated from pooled human serum.
• Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab’)2 part of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
  The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: FITC
  State: Lyophilized purified IgG fraction
  Label: Fluorescein Isothiocyanate
  Absorption emission: 492 nm / 515 nm
  Molar radio: Fluorochrome/IgG ~1.0
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Conjugation: FITC
• Storage: Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated thawing and freezing.