Human IgG4 (pFC specific) Mouse Monoclonal Antibody [Clone ID: NI 315 (HP 6206)]

CAT#: AM20257FC-N

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), FITC

Specifications

Product Data

• Clone Name: NI 315 (HP 6206)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
  General Recommended Dilutions:
  Histochemical Use: 1/10-1/50.
  ELISA: from 1/200 upwards.
  Western blot: from 1/400 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgG4 isolated from Human serum.
• Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
  The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: FITC
  State: Lyophilized purified IgG fraction
  Label: Fluorescein Isothiocyanate
  Absorption emission: 492 nm / 515 nm
  Molar radio: Fluorochrome/IgG ~1.5
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Conjugation: FITC
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.