Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]

CAT#: AM20258TC-N

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, TRITC


USD 680.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name NI 335 and NI 343
Applications ELISA, IF, IP, WB
Recommended Dilution To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to etermine its concentration in techniques as ELISA, indirect mmunoperoxidase staining of ytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/1-1/50.
ELISA: from 1/20 upwards.
Western blot: from 1/40 upwards.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified polyclonal IgG isolated from human serum.
Specificity The reactivity of the this monoclonal antibody is restricted to isotype specific determinants on the Fc part of IgG. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins.
The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: TRITC
State: Lyophilized purified IgG fraction
Label: Tetramethylrhodamine Iothiocyanate
Absorption emission: 554 nm / 573 nm
Molar radio: Peroxidase/IgG ~1.8
Reconstitution Method Restore by adding 0.5 ml sterile distilled water
Concentration 0.4 mg/ml
Conjugation TRITC
Storage Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer.
Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Avoid repeated thawing and freezing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.