Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]

CAT#: AM20259AF-N

Human IgM mouse monoclonal antibody, clone NI 179, Ascites

Specifications

Product Data

• Clone Name: NI 179
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. The optimum working
  dilution is an assay-related characteristic and should always be determined by titration.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/200
  ELISA: from 1/500 upwards
  Western blot: from 1/1,000 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgM isolated from pooled Human serum
• Specificity: The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
  The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in Indirect Binding Enzyme Immunoassay, Immunoblotting, Immunoprecipitation and Indirect Immunoperoxidase staining of cytoplasmic Ig.
• Formulation: State: Ascites
  State: Lyophilized (Delipidated, heat inactivated) stable whole ascites without preservatives and foreign proteins.
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
• Concentration: 1.0 mg/ml
• Storage: Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated thawing and freezing.