Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]

CAT#: AM20259BT-N

Human IgM mouse monoclonal antibody, clone NI 179, Biotin

Specifications

Product Data

• Clone Name: NI 179
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. As a second step an Avidin or Streptavidin conjugate have to be used.
  General Recommended Dilutions:
  Histochemical Use: 1/100-1/500
  ELISA: from 1/1,000 upwards
  Western blot: from 1/2,000 upwards
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgM isolated from pooled Human serum
• Specificity: The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in Indirect Binding Enzyme Immunoassay, Immunoblotting, Immunoprecipitation and Indirect Immunoperoxidase staining of cytoplasmic Ig.
  The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Molar radio: Biotin/IgG: ~7.1
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Storage: Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated thawing and freezing.
• Note: Conjugation Procedure: Conjugation is carried out using a proprietary technique for the binding of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life