Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]

CAT#: AM20259FC-N

Human IgM mouse monoclonal antibody, clone NI 179, FITC

Specifications

Product Data

• Clone Name: NI 179
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
  General Recommended Dilutions:
  Histochemical Use: 1/10-1/50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgM isolated from pooled Human serum
• Specificity: The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
  The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in Indirect Binding Enzyme Immunoassay, Immunoblotting, Immunoprecipitation and Indirect Immunoperoxidase staining of cytoplasmic Ig.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: FITC
  State: Lyophilized purified IgG fraction
  Label: Fluorescein Isothiocyanate Conjugation Procedure: A proprietary technique for the binding to is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life
  Absorption emission: 492 nm / 515 nm
  Molar radio: Fluorochrome/IgG: ~1.4
• Concentration: 0.4 mg/ml
• Purification: Immunoaffinity Chromatography
• Conjugation: FITC
• Storage: Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated thawing and freezing.