Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]

CAT#: AM20259HR-N

Human IgM mouse monoclonal antibody, clone NI 179, HRP

Specifications

Product Data

• Clone Name: NI 179
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Dot blot.
  Immunoblotting.
  Immunocytochemistry.
  Immunohistochemistry on Paraffin Sections.
  To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/200.
  ELISA: from 1/250 upwards.
  Western blot: from 1/500 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgM isolated from pooled Human serum
• Specificity: The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in Indirect Binding Enzyme Immunoassay, Immunoblotting, Immunoprecipitation and Indirect Immunoperoxidase staining of cytoplasmic Ig.
  The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without foreign proteins
  Label: HRP
  State: Lyophilized purified IgG fraction
  Preservative: None as it may interfere with the antibody activity
  Label: Horseradish Peroxidase enriched for isoenzyme C (RZ=3.2)
  Molar radio: Peroxidase/IgG: ~1.7
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Purification: Immunoaffinity Chromatography
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Note: Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life