Human IgA (Secretory component) Mouse Monoclonal Antibody [Clone ID: NI 194-4 (A89-039)]

CAT#: AM20261BT-N

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Biotin

Specifications

Product Data

• Clone Name: NI 194-4 (A89-039)
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
  General Recommended Dilutions:
  Histochemical Use: from 1/20 upwards
  ELISA: form 1/25 upwards
  Western blot: from 1/50 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified Secretory component isolated from Human milk.
• Specificity: The antiserum reacts with Human secretory component, free and bound. It does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
  The reactivity of the antiserum is restricted to Secretory component as tested in Indirect Binding Immunoblotting and Indirect Immunoperoxidase staining.
• Formulation: PBS, pH 7.2 without preservatives.
  No foreign protein added.
  Label: Biotin
  State: Lyophilized purified IgG fraction.
  Label: Marker: N-Hydroxysuccinimidobiotin. Conjugation procedure: Conjugation is carried out using a proprietary technique for the coupling of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life
  Molar radio: Biotin/IgG: ~1.4
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
  Dilutions may be prepared by adding PBS, pH 7.2
• Concentration: 0.4 mg/ml
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.