Human IgA (IgAc, A1, A2, Secretory) Mouse Monoclonal Antibody [Clone ID: NI 69 and NI 184+NI 69-11+NI 512+NI 194]
CAT#: AM31706AF-N
Human IgA (IgAc, A1, A2, Secretory) mouse monoclonal antibody, clone NI 69 and NI 184+NI 69-11+NI 512+NI 194, Ascites
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Specifications
Product Data | |
Clone Name | NI 69 and NI 184+NI 69-11+NI 512+NI 194 |
Applications | ELISA, IF, IHC, IP, R, WB |
Recommended Dilution | Can be used to identify the presence of IgA and secretory subclasses in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting. Recommended Working Dilutions: Histochemical Use: 1/100-1/500. ELISA: from 1/1000 Upwards. Western blotting: 1/2000-1/10000. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Highly purified monoclonal IgA subclasses isolated from pooled Human serum, secretory components isolated from Human milk. |
Specificity | The reactivity of the antibody is restricted to the given protein as tested in Indirect Binding Enzyme Immunoassay, Immunoblotting, Immunoprecipitation and Indirect Immunoperoxidase staining of cytoplasmic Immunoglobulins. Cross-reactivity: The antibodies do not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. These antisera have not been tested for cross-reactivity with other species. |
Formulation | State: Ascites State: Delipidated, heat inactivated, lyophilized stable ascites |
Reconstitution Method | Restore with 0.5 ml sterile distilled water. |
Concentration | 1.0 mg/ml (after reconstitution) |
Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Note | This Set contains 4 different Monoclonal Antibodies: Cat.-No AM20249AF-N Human IgA (clone NI 69 (A89-034) and NI 184 (A89-035)) Cat.-No AM20246AF-N: Clone NI 69-11 (A89-036) Cat.-No AM20247AF-N: Clone NI 512 (A89-038) Cat.-No AM20261SU-N: Clone NI 194-4 (A89-039) |
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