Human IgG, IgA, IgM, IgD, IgE Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307]
CAT#: AM31707AF-N
Human IgG, IgA, IgM, IgD, IgE mouse monoclonal antibody, clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307, Ascites
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Specifications
Product Data | |
Clone Name | NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307 |
Applications | ELISA, IF, IHC, IP, R, WB |
Recommended Dilution | Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting. Recommended Working Dilutions: Histochemical Use: 1/100-1/500. ELISA: from 1/1000 Upwards. Western blotting: 1/2000 Upwards. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Highly purified monoclonal IgG subclasses isolated from pooled human serum. |
Specificity | The reactivity of the antibody is restricted to a isotype specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins. Cross-reactivity: The antibodies do not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. These antisera have not been tested for cross-reactivity with other species. |
Formulation | State: Ascites State: Delipidated, heat inactivated, lyophilized stable ascites. |
Reconstitution Method | Restore with 0.5 ml sterile distilled water. |
Concentration | 1.0 mg/ml (after reconstitution) |
Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Note | This Set contains 5 different Monoclonal Antibodies: Cat.-No AM20258AF-N Human IgG (clone NI 335 and NI 343) Cat.-No AM20259AF-N Human IgM (clone NI 179) Cat.-No AM20249AF-N Human IgA (clone NI 69 (A89-034) and NI 184 (A89-035)) Cat.-No AM20250AF-N Human IgD (clone NI 158) Cat.-No AM20251AF-N Human IgE (clone NI 307) |
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