Human IgG (heavy chain) Mouse Monoclonal Antibody [Clone ID: SPM556]

CAT#: AM50118PU-T

Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified


USD 240.00

2 Weeks*

Size
    • 20 ug

Product Images

Specifications

Product Data
Clone Name SPM556
Applications FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Purified Human Ig Gamma Chain.
Specificity Recognizes a protein of 75kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Cellular Localization: Cytoplasm, Cell Surface and Secreted.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration 0.2 mg/ml
Purification Protein A/G Chromatography
Storage Store undiluted at 2-8°C.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.