Chicken IgM (Fc specific) Goat Polyclonal Antibody

CAT#: AP21443SU-N

Chicken IgM (Fc specific) goat polyclonal antibody, Texas Red

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in chicken serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of chicken IgM from serum or plasma.
  Recommended Working Dilutions:
  Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
  In double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
• Reactivities: Chicken
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly purified normal IgM isolated from pooled chicken serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal chicken serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments.
  Cross-Reactivity: The antiserum does not cross-react with any other component of the chicken Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.
• Formulation: Label: Texas Red
  State: Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives
  Label: Labeled with Texas Red.
• Reconstitution Method: Restore by adding 1 ml of sterile distilled water.
• Concentration: Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
• Conjugation: Texas Red
• Storage: Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.