Equine IgG (Fc specific) Goat Polyclonal Antibody

CAT#: AP21448SU-N

Equine IgG (Fc specific) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in horse serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgG from serum or plasma.
  Directions for use:
  Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
  Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
  Antibody titre: Precipitin titre 1:32 when tested against pooled normal horse serum in agar-block immunodiffusion titration.
• Reactivities: Equine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly purified normal IgG isolated from pooled horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to the Fc part of the IgG molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal horse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments.
  Cross-reactivity: The antiserum does not cross-react with any other component of the horse immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: State: Serum
  State: Lyophilized, Delipidated, Heat inactivated, Stable Whole Serum without preservatives
• Reconstitution Method: Restore by adding 1 ml of sterile distilled water
• Concentration: Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
• Storage: Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.