Equine IgG1 Goat Polyclonal Antibody

CAT#: AP21449SU-N

Equine IgG1 goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG1 in Horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgG1 from serum or plasma.
  Directions for Use:
  Immunoelectrophoresis: use 2 µl or equivalent against 120 µl antiserum.
  Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
  Antibody titre: Precipitin titre not less then 1/32 when tested against pooled normal horse serum in agar block immunodiffusion titration.
• Reactivities: Equine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Pools of purified normal IgG1 isolated from pooled serum from tetanus immunized Horses.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of subclass IgG1. It does not react with IgG/Fab fragments, IgM and IgA or any non-Ig protein in horse serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity: This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.
• Formulation: State: Serum
  State: Lyophilized, Delipidated, Heat inactivated, Stable Whole Serum without preservatives
• Reconstitution Method: Restore by adding 1 ml of sterile distilled water
• Concentration: Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
• Storage: Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.