Human IgA (Secretory component) Goat Polyclonal Antibody

CAT#: AP21476BT-N

Human IgA (Secretory component) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP, WB
• Recommended Dilution: This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Human body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended Working Dilutions:
  Histochemical and Cytochemical Use: 1/100-1/500
  ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified free Human Secretory component isolated from pooled milk is used for immunization.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Tested in Immunoelectrophoresis, Double Radial Immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in Human secretions.
  In Immunoelectrophoresis against Human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region.
  The Biotin immunoconjugate does not react with other molecular forms of IgA, or with any other Secretory or plasma protein.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized purified hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~4.5
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water.
• Concentration: 10.0 mg/ml
• Purification: Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and purification of the IgG fraction by DEAE-Chromatography.
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.