Human IgA (Secretory component) Goat Polyclonal Antibody

CAT#: AP21476FC-N

Human IgA (Secretory component) goat polyclonal antibody, FITC

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP
• Recommended Dilution: Can be used as reagent for the direct detection of secretory component in human cells, tissues and body fluids in Immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
  This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Working Dilutions: 1/20-1/80.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified free Human Secretory component isolated from pooled milk is used for immunization.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Tested in Immunoelectrophoresis, Double Radial Immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in Human secretions.
  In Immunoelectrophoresis against Human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The Biotin immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: FITC
  State: Lyophilized purified hyperimmune IgG fraction
  Label: Fluorescein Isothiocyanate isomer 1 (FITC)
  Absorption emission: 492 nm / 515 nm
  Molar radio: Fluorescein/IgG ~1.6
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10 mg/ml
• Purification: Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and purification of the IgG fraction by DEAE-Chromatography.
• Conjugation: FITC
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.