Human IgA (Secretory component) Goat Polyclonal Antibody

CAT#: AP21476HR-N

Human IgA (Secretory component) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP, WB
• Recommended Dilution: In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in human milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended Working Dilutions:
  Histochemical and Cytochemical Use: 1/100-1/500.
  ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/6000.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Secretory component can be present in Human secretions bound to secretory IgA (sIgA) and in free form.
  Free Human secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal human milk, using a high electroendosmosis agar plate, free secretory component is precipitated in the alpha-2 region. This antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein and double radial immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent of the concentration of the reactants and he sensitivity of the method, but has not been tested in detail.
• Formulation: PBS, pH 7.2 without preservatives.
  No foreign protein added
  Label: HRP
  State: Lyophilized purified IgG fraction
  Label: Horseradish Peroxidase
  Molar radio: Peroxidase/IgG: ~1.7
• Reconstitution Method: Restore by adding 1.0 ml sterile distilled water.
  Dilutions may be prepared by adding PBS, pH 7.2
• Concentration: 10.0 mg/ml
• Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography
• Conjugation: HRP
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
  
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.