Mouse IgD (Fc specific) Goat Polyclonal Antibody

CAT#: AP21481AF-N

Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, WB
• Recommended Dilution: Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
  Recommended Working Dilutions:
  Histochemical Use: 1/50-1/250.
  ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
• Reactivities: Mouse
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified polyclonal and monoclonal IgD isolated from BALB/C and C57BL Mouse serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgD molecule which expresses strict isotypic (class) specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against serum of mice belonging to different allotypic groups, a single precipitin line is obtained which show a reaction of identity with the precipitin lines obtained with the purified IgD of BALB/C and C57BL origin used as immunogens. It does not react with IgG, including all subclasses, IgM and IgG/Fab or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also reacts with membrane-bound IgD in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.
• Formulation: PBS, pH 7.2 without preservatives
  State: Azide Free
  State: Lyophilized purified Hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: DEAE Chromatography
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.