Mouse IgE (Fc specific) Goat Polyclonal Antibody

CAT#: AP21482HR-N

Mouse IgE (Fc specific) goat polyclonal antibody, HRP


USD 550.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Applications ID, IF, IHC, IP, WB
Recommended Dilution In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
depending on the method used.
Reactivities Mouse
Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Purified homogenous IgE isolated from pooled Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule which expresses strict isotypic (class) specificity as tested against mouse serum and a panel of purified homogenous immunoglobulins.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: HRP
State: Lyophilized purified IgG fraction
Label: Horseradish Peroxidase. 
Enzyme marker: Horseradish peroxidase enriched for isoenzyme C (RZ=3.2). 
Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate technique for the binding to peroxidase, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life
Molar radio: Peroxidase/IgG: ~1.7
Reconstitution Method Restore by adding 1.0 ml sterile distilled water
Purification Immunoaffinity Chromatography
Conjugation HRP
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Note Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.um

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.