Rabbit IgM (Fc specific) Goat Polyclonal Antibody

CAT#: AP21488HR-N

Rabbit IgM (Fc specific) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP, WB
• Recommended Dilution: Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids.
  This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended Working Dilutions:
  Histochemical and Cytochemical Use: 1/100-1/500.
  ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
• Reactivities: Rabbit
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified normal IgM isolated from pooled Rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: HRP
  State: Lyophilized purified Hyperimmune IgG fraction
  Label: Horseradish Peroxidase
  Molar radio: Peroxidase/IgG: ~1.7
• Reconstitution Method: Restore by adding 1.0 ml sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: DEAE Chromatography
• Conjugation: HRP
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.