Monkey IgA (Fc specific) Goat Polyclonal Antibody

CAT#: AP31437HR-N

Monkey IgA (Fc specific) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP, WB
• Recommended Dilution: Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in monkey serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended working dilutions:
  For histochemical and cytochemical use are usually between 1/100 and 1/500.
  In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
• Reactivities: Monkey
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified normal IgA isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with IgA in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react weakly with IgA of other species as has been observed for chimpanzee and man.
• Formulation: PBS, pH 7.2
  No preservative added, as it may interfere with the antibody activity. 1.7. No foreign proteins added.
  Label: HRP
  State: Lyophilised hyperimmune Ig fraction
  Label: Horseradish Peroxidase
  Molar radio: 1,7
• Reconstitution Method: Restore with 1 ml sterile distilled water.
• Concentration: 10 mg/ml
• Purification: DEAE-column Chromatography
• Conjugation: HRP
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.