Monkey IgG (Fc specific) Goat Polyclonal Antibody

CAT#: AP31438AF-N

Monkey IgG (Fc specific) goat polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, WB
• Recommended Dilution: As Unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
  To prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
  When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
  Recommended Working Dilutions:
  Histochemical Use: 1/50-1/250.
  ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
  Antibody Titre: Precipitin titre not less than 1/32 when tested against pooled normal Rhesus Monkey serum in agar-block immunodiffusion titration.
• Reactivities: Monkey
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified normal IgG isolated from pooled Rhesus Monkey serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in Monkey serum, as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with IgG in serum of other Old-World Monkeys, including Cercopithecus, Cynomolgus and Baboon. The product may also react with IgG of other species as has been observed for Chimpanzee and Man.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins
  State: Azide Free
  State: Lyophilized purified Hyperimmune IgG fraction
• Reconstitution Method: Restore with 1.0 ml sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: DEAE-column Chromatography
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.