Monkey IgM (Fc specific) Goat Polyclonal Antibody

CAT#: AP31440TC-N

Monkey IgM (Fc specific) goat polyclonal antibody, TRITC

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP
• Recommended Dilution: Can be used for direct immunofluorescence staining of cytoplasmic IgM of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Working dilutions are usually between 1/10 and 1/40.
• Reactivities: Monkey
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified polyclonal IgM and pools of homogenous IgM isolated from pooled monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The product may also react with other species as has been observed for chimpanzee and man.
• Formulation: PBS, pH 7.2
  No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
  Label: TRITC
  State: Lyophilised hyperimmune Ig fraction
  Label: Tetramethylrhodamine isothiocyanate isomer R
  Absorption emission: 554 nm / 573 nm
  Molar radio: 1,3
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Concentration: 10 mg/ml
• Purification: DEAE-column Chromatography
• Conjugation: TRITC
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.