Monkey IgG (Fab specific) Rabbit Polyclonal Antibody

CAT#: AP31444HR-N

Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP, WB
• Recommended Dilution: Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells.
  Combinations of isotype-specific reagents should be used instead for this purpose.
  This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommneded Working Dilutions:
  Histochemical and Cytochemical Use: 1/100-1/500.
  ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000.
• Reactivities: Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified normal Fab of polyclonal Rhesus Monkey IgG.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits, and with free light chains of kappa and lambda type. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled monkey serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail, however in double radial immunodiffusion (Ouchterlony) a reaction has been obtained with human immunoglobulins.
• Formulation: PBS, pH 7.2, No preservative added, as it may interfere with he antibody activity. No foreign proteins added.
  Label: HRP
  State: Lyophilized purified Hyperimmune IgG fraction
  Stabilizer: None
  Preservative: None
  Label: Horseradish Peroxidase
  Molar radio: 1.7
• Reconstitution Method: Restore with 1 ml sterile distilled water.
• Concentration: 10.0 mg/ml
• Purification: DEAE-column Chromatography
• Conjugation: HRP
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.