Monkey IgA (Fc specific) Rabbit Polyclonal Antibody

CAT#: AP31445FC-N

Monkey IgA (Fc specific) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP
• Recommended Dilution: In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Working dilutions: are usually between 1:20 and 1:80, depending on the method used.
• Reactivities: Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Pools of purified IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against monkey serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgA used as immunogens. It does not react with IgG, IgG/Fab fragments and IgM or any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for chimpanzee and man.
• Formulation: PBS, pH 7.2
  No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
  Label: FITC
  State: Lyophilised hyperimmune Ig fraction
  Label: Fluorescein isothiocyanate isomer 1
  Absorption emission: 492nm/515nm
  Molar radio: 3,9
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Concentration: 10 mg/ml
• Conjugation: FITC
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.