Monkey IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: AP31448SU-N

Monkey IgG (H+L chain) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: IP
• Recommended Dilution: Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal monkey serum; to prepare an immunoadsorbent for the production of Ig-free monkey serum, plasma or non-Ig preparations.
  Recommended dilutions:
  In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
  In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
• Reactivities: Monkey
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified normal IgG isolated from pooled rhesus monkey serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with IgG in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with IgG of other species as has been observed for chimpanzee and man. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results:
  ++ Human
  + bovine, cat, dog, goat, guinea pig, hamster, horse, mouse, rat, sheep, swine
  - chicken, pigeon, turkey
• Formulation: No preservative added, as it may interfere with the antibody activity. No foreign protein added.
  State: Serum
  State: Delipidated, heat inactivated lyophilized stable whole serum
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Antibody titre: Precipitin titre not less than 1/64 when tested against normal monkey serum in agar block titration.