Monkey IgA (Secretory component) Goat Polyclonal Antibody

CAT#: AP31450AF-N

Monkey IgA (Secretory component) goat polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, WB
• Recommended Dilution: Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes.
  As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
  Working dilutions:
  Histochemistry: 1/50 - 1/250.
  ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/1000.
• Reactivities: Chimpanzee, Monkey
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The antiserum reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal monkey milk, using a high electroendosmosis agar plate, free secretory component ism precipitated in the alpha-2 region. This antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein and double radial immunodiffusion.
  
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent of the concentration of the reactants and he sensitivity of the method. This product may also react with free and bound secretory component in many old-world monkeys, including Macaca Sylvanus, Cercopithecus, Cynomolgus, Baboon, and also with the secretory component of chimpanzees. In double radial immunodiffusion a reaction has been observed with free human secretory component.
• Formulation: PBS, pH 7.2
  No preservative added, as it may interfere with the antibody activity. No foreign protein added.
  State: Azide Free
  State: Lyophilised hyperimmune Ig fraction
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Concentration: 10 mg/ml
• Purification: DEAE-column Chromatography
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
  Antibody titre: Precipitin titre not less than 1/16 when tested against normal monkey milk in agar block titration.