Monkey IgA (Secretory component) Goat Polyclonal Antibody

CAT#: AP31450SU-N

Monkey IgA (Secretory component) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, ID, IP
• Recommended Dilution: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
  Can be used in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in monkey serum or other body fluids and to determine its concentration.
  Recommended dilutions:
  Immunoelectrophoresis: use 2 μl serum or equivalent against 120 μl antiserum.
  Double radial immunodiffusion: use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
• Reactivities: Chimpanzee, Monkey
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free secretory component isolated from pooled rhesus monkey milk is used for immunization.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein.
  Cross-reactivity
  Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The antiserum may also react with other species as has been observed for chimpanzee.
• Formulation: Without preservatives and foreign proteins.
  State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole antiserum
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
  Antibody titre Precipitin titre 1:64 when tested against pooled normal monkey milk in agar-block immunodiffusion titration.