Feline IgG (H+L chain) Goat Polyclonal Antibody

CAT#: AP31537SU-N

Feline IgG (H+L chain) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP, R
• Recommended Dilution: Can be used:
  • In precipitating techniques as Immunoelectrophoresis and Double Radial Immunodiffusion to identify the presence of IgG.
  • As secondary antibody to precipitate the immunoglobulin in normal Cat serum.
  • To prepare an immunoadsorbent for the production of Ig-free Cat serum, plasma or non-Ig preparations.
  Directions for use:
  Immunoelectrophoresis: Use 2 μl or equivalent against 120 μl antiserum.
  Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
• Reactivities: Feline
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified normal IgG isolated from pooled cat serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in Cat serum, as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: No preservative added, as it may interfere with the antibody activity. No foreign protein added.
  State: Serum
  State: Delipidated, heat inactivated lyophilized stable whole serum
• Reconstitution Method: Restore with 1 ml sterile distilled water.
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.