Feline IgG (H+L chain) Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ID, IP, R |
Recommended Dilution | Can be used: • In precipitating techniques as Immunoelectrophoresis and Double Radial Immunodiffusion to identify the presence of IgG. • As secondary antibody to precipitate the immunoglobulin in normal Cat serum. • To prepare an immunoadsorbent for the production of Ig-free Cat serum, plasma or non-Ig preparations. Directions for use: Immunoelectrophoresis: Use 2 μl or equivalent against 120 μl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
Reactivities | Feline |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Purified normal IgG isolated from pooled cat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in Cat serum, as tested by Immunoelectrophoresis and Double Radial Immunodiffusion. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. |
Formulation | No preservative added, as it may interfere with the antibody activity. No foreign protein added. State: Serum State: Delipidated, heat inactivated lyophilized stable whole serum |
Reconstitution Method | Restore with 1 ml sterile distilled water. |
Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
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