Canine IgG (H+L chain) Goat Polyclonal Antibody

CAT#: AP31541FC-N

Canine IgG (H+L chain) goat polyclonal antibody, FITC


USD 480.00

2 Weeks*

Size
    • 2 ml

Product Images

Specifications

Product Data
Applications ELISA, IF, IHC, IP
Recommended Dilution Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/120.
Reactivities Canine
Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation PBS, pH 7.2
No preservative added - No foreign proteins added
Label: FITC
State: Lyophilised hyperimmune Ig fraction
Label: Fluorescein isothiocyanate isomer 1
Absorption emission: 492nm / 515nm
Molar radio: 1,6
Reconstitution Method Restore with 2 ml sterile distilled water
Concentration 10 mg/ml
Purification DEAE-column Chromatography
Conjugation FITC
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.