Canine IgG (H+L chain) Goat Polyclonal Antibody

CAT#: AP31541SU-N

Canine IgG (H+L chain) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal dog serum; to prepare an immunoadsorbent for the production of Ig-free dog serum, plasma or non-Ig preparations.
  Directions for use:
  In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum.
  In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells.
• Reactivities: Canine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: No preservative added, as it may interfere with the antibody activity. No foreign protein added.
  State: Serum
  State: Delipidated, heat inactivated lyophilized stable whole serum
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.