Human IgG Rabbit Polyclonal Antibody

CAT#: AP31649PU-N

Human IgG rabbit polyclonal antibody, Ig Fraction

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Immunoprecipitation.
  Immunodiffusion.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Specificity: This antibody is directed against Human IgG (heavy and light chains).
  This IgG fraction was isolated from a high titer antiserum by salt fractionation and DEAE ion exchange chromatography. It was titered against its immunogens, sterile filtered, and analyzed for immunoglobulin purity and protein concentration.
• Formulation: Sterile PBS, pH 7.8
  State: Ig Fraction
  State: Liquid purified Ig fraction
• Concentration: 9.7 mg/ml
• Purification: Salt Fractionation and DEAE Ion Exchange Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Note: Ouchterlony: Results
  Test #1
  Sample Size: 10 µl
  Distance Between Wells: 5 mm
  Test time: 24 hours
  Temperature: 37°C
  Sample tested: Normal human serum at dilutions of 1/4 to 1/8
  Results: One band at all dilutions
  
  Test #2
  Sample Size: 10 µl
  Distance Between Wells: 5 mm
  Test time: 24 hours
  Temperature: 37°C
  Sample tested: Human IgG, 5 mg/ml
  Results: One band homogeneous with band in test #1
  
  Sterility Test: Thioglycollate Broth @ 37°C, Trypticase Soy Broth @ 37°C, Sabouraudss Broth @ 22°C
  Results: No growth.