Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: AP31662PU-N

Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Immunoprecipitation.
  Immunodiffusion.
  Ouchterlony Test:
  Sample size: 15 µl.
  Distance between wells: 5 mm.
  Test Time: 5 days.
  Temperature: 37°C.
  Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG.
  Results:
  Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG.
  No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution.
  Protein content: Biuret Assay 14 mg/ml.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Specificity: This antibody reacts to Mouse IgG (H&L).
  This is Rabbit IgG directed against Mouse IgG (heavy and light chains). This IgG fraction was isolated from a high titer antiserum by salt fractionation and DEAE ion exchange chromatography. It was titered against its immunogens, sterile filtered, and analyzed for immunoglobulin purity and protein concentration.
• Formulation: PBS, sterile pH 7.8
  State: Ig Fraction
  State: Liquid purified IgG fraction
• Purification: Salt Fractionation and DEAE Ion Exchange Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.