Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

CAT#: AP31795AP-N

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate.
  Western Blot (1/1000).
  Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
  Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
• Reactivities: Chicken
• Host: Goat
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
• Specificity: Chicken IgY (heavy and light chains).
• Formulation: 50mM HEPES, pH 7.0, 0.1M NaCl, 1.0mM MgCl2, 0.1mM ZnCl2 containing 0.2% BSA as stabilizer and 0.02% Sodium Azide as preservative
  Label: AP
  State: Liquid purified IgY fraction
  Label: Alkaline Phosphatase
• Concentration: 0.1 mg/ml (based on absorbance at 280 nm)
• Purification: Affinity Chromatography
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C in the dark.