Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

CAT#: AP31795FC-N

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Immunocytochemistry (1/200).
  In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS).
  Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
• Reactivities: Chicken
• Host: Goat
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
• Specificity: Chicken IgY (heavy and light chains).
• Formulation: PBS, pH 7.2 containing 0.2% BSA as stabilizer and 0.02% Sodium Azide as preservative
  Label: FITC
  State: Liquid purified IgY fraction
  Label: Fluorescein Isothiocyanate
  Molar radio: F/P = 3.2
• Concentration: 1.0 mg/ml (based on absorbance at 280 nm)
• Purification: Affinity Chromatography
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C in the dark.