Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

CAT#: AP31795PU-N

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution).
  Western blot (1/5000).
  Immunocytochemistry.
  Immunohistochemistry (1/250).
  Immunoprecipitation (1/250).
  Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
• Reactivities: Chicken
• Host: Goat
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
• Specificity: Chicken IgY (heavy and light chains).
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified IgY fraction
  Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml (based on a Bradford Assay)
• Purification: Affinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C in the dark for one month or Add Sterile Glycerol and store at -20°C for longer.
  Avoid repeated freezing and thawing.
  Centrifuge vial before opening.