Goat IgG (H+L chain) Chicken Polyclonal Antibody

CAT#: AP31796FC-N

Goat IgG (H+L chain) chicken polyclonal antibody, FITC

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunocytochemistry (1/1000).
  Immunohistochemistry (1/250).
  Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
• Reactivities: Goat
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
  Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated
  with Fluorescein isothiocyanate.
• Specificity: Goat IgG (heavy and light chains).
  There is no detectable cross-reactivity with Rabbit, Human or Mouse IgG.
• Formulation: PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein
  Absorption emission: Ratio of absorbance at 495/280 = 1.1
  Molar radio: F/P = 5.6
• Concentration: 1.0 mg/ml (based on absorbance at 280 nm)
• Purification: Affinity Chromatography.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C in the dark.