Goat IgG (H+L chain) Chicken Polyclonal Antibody

CAT#: AP31796HR-N

Goat IgG (H+L chain) chicken polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, ID, WB
• Recommended Dilution: ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
  Western Blot (1/5000).
  Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis.
• Reactivities: Goat
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
  Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.
• Specificity: Goat IgG (heavy and light chains).
  There is no detectable cross-reactivity with Rabbit, Human or Mouse IgG.
• Formulation: 50mM HEPES, pH 7.0 containing 0.1M NaCl
  Label: HRP
  State: Liquid purified IgY fraction
  Stabilizer: 0.2% BSA
  Preservative: 0.01% Thimerosal
  Label: Horseradish Peroxidase
• Concentration: 1.0 mg/ml (based on absorbance at 280 nm)
• Purification: Affinity Chromatography
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C in the dark.