Human IgG (H+L chain) Chicken Polyclonal Antibody

CAT#: AP31797FC-N

Human IgG (H+L chain) chicken polyclonal antibody, FITC

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunocytochemistry.
  Immunohistochemistry (1/250).
  Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
• Reactivities: Human
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Purified, non-denatured Human IgG (containing both heavy and light chains).
  Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate.
• Specificity: Human IgG (heavy and light chains).
  There is no detectable cross-reactivity with Goat, Mouse or Rabbit IgG.
• Formulation: PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein
  Absorption emission: Ratio of absorbance at 495/280 = 1.1
  Molar radio: F/P = 5.6
• Concentration: 1.0 mg/ml (based on absorbance at 280 nm)
• Purification: Affinity Chromatography.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C in the dark.