Mouse IgG (H+L chain) Chicken Polyclonal Antibody

CAT#: AP31798HR-N

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, ID, WB
• Recommended Dilution: ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
  Western Blot: 1/200 -1/5000.
  Immunocytochemistry.
  Immunohistochemistry: 1/250 - 1/5000.
  Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
• Reactivities: Mouse
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Purified, non-denatured Mouse IgG (containing both heavy and light chains).
  Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase.
• Specificity: Mouse IgG (heavy and light chains).
  There is no detectable cross-reactivity with Goat, Human or Rabbit IgG.
• Formulation: 10 mM Sodium Phosphate, pH 7.2, 0.15 M Sodium Chloride
  Label: HRP
  State: Liquid purified Ig fraction.
  Stabilizer: 10 % (w/v) BSA (Protease and IgG free)
  Preservative: 0.1 % (v/v) Kathon CG
  Label: Horseradish Peroxidase
• Concentration: 1.0 mg/ml (based on absorbance at 280 nm)
• Purification: Affinity Chromatography
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C in the dark.