Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody

CAT#: AP32278RP-S

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Product Images

Immunofluorescent Staining: Human peripheral blood mononuclear cells were labeled with either Mouse anti-Human CD3 (IgG1) (A), anti-Human CD5 (IgG2a) (B), anti-Human CD22 (IgG2b) (C), anti-Human IgD (IgG3) (D) or anti-Human CD57 (IgM) (E). After washing the cells were then stained with Goat anti-Mouse Ig-R-PE Cat.-No AP32278RP (0.1 µg/106 cells), following which lymphocytes were gated and analyzed by Flow Cytometry.

Specifications

Product Data

• Applications: ELISA, FC, IF, IHC
• Recommended Dilution: FLISA: ? 0.1 µg/ml
  Flow Cytometry: ? 0.1 µg/10⁶ cells.
  
• Reactivities: Mouse
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Goats were hyperimmunized with Mouse IgM, IgG and IgA.
• Specificity:

  This antibody reacts with the heavy and light chains of Mouse IgM, IgG1, IgG2a, IgG2b,IgG2c, IgG3 and IgA.
  Cross Absorption: Human immunoglobulins and pooled sera.
  May react with immunoglobulins from other species.

• Formulation: PBS containing 0.09% Sodium Azide and a stabilizing agent
  Label: PE
  State: Liquid purified Ig fraction
  Label: R-phycoerythrin (R-PE)
• Concentration: 0.5 mg/ml
• Purification: Affinity Chromatography on pooled mouse IgM+IgG+IgA covalently linked to Agarose
• Conjugation: PE
• Storage:

  Store undiluted at 2-8°C.
  DO NOT FREEZE!
  Protect from light!
  

• Note: Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA, FLISA, and/or Flow Cytometry to conform to characteristics of a standard reference reagent.