Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: AP33082BT-N

Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Dot blot.
  Immunoblotting.
  Immunocytochemistry.
  Immunohistochemistry Paraffin Sections.
  Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended Working Dilutions:
  Histochemistry and Cytochemical Use: 1/100-1/500.
  ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
• Reactivities: Sheep
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified normal IgG isolated from pooled sheep serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in sheep serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized purified hyperimmune IgG fraction
  Stabilizer: None
  Preservative: None, as it may interfere with the antibody activity.
  Label: N-Hydroxysuccinimidobiotin
  Molar radio: Biotin/IgG ~4.2
• Reconstitution Method: Restore with 1 ml sterile distilled water.
• Concentration: 10.0 mg/ml
• Purification: Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Not required