Sheep IgM (Fc specific) Rabbit Polyclonal Antibody

CAT#: AP33083BT-N

Sheep IgM (Fc specific) rabbit polyclonal antibody, Biotin


USD 550.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Applications ELISA, IF, IHC, WB
Recommended Dilution ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/4,000.
Reactivities Sheep
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in sheep serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however a strong reaction with goat IgM has been observed.
Formulation PBS, pH 7.2
Label: Biotin
State: Lyophilized purified hyperimmune IgG fraction
Stabilizer: None
Preservative: None, as it may interfere with the antibody activity.
Label: N-Hydroxysuccinimidobiotin
Molar radio: Biotin/IgG ~4.6
Reconstitution Method Restore with 1 ml sterile distilled water.
Concentration 10.0 mg/ml
Purification Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Note Adsorption: Not required

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.