Porcine IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: AP33087AF-N

Porcine IgG (H+L chain) rabbit polyclonal antibody, Azide Free


USD 180.00

2 Weeks*

Size
    • 10 mg

Product Images

Specifications

Product Data
Applications ELISA, IF, WB
Recommended Dilution ELISA. 
Dot blot.
Immunoblotting.
Indirect Immunofluorescence. 

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal Swine serum in agar-block immunodiffusion titration.
Reactivities Porcine
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in swine serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: Bovine: +, Cat: +, Chicken: -, Dog: +, Duck: -, Goat: +, Guinea pig: +, Human: +, Horse: +, Mouse: +, Monkey: +, Pigeon: -, Rat: +, Sheep: +, Turkey: -.
Formulation PBS, pH 7.2
State: Azide Free
State: Lyophilized purified hyperimmune IgG fraction
Stabilizer: None
Preservative: None, as it may interfere with the antibody activity.
Reconstitution Method Restore with 1 ml sterile distilled water.
Concentration 10.0 mg/ml
Purification Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Note Adsorption: Not required.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.