Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) Goat Polyclonal Antibody
CAT#: AP33463AP-N
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, AP, Aff - Purified
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Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommended Dilution | Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Recommended Dilutions: ELISA: 1/10,000-1/40,000. Western Blot: 1/200-1/2,000. Immunohistochemistry: 1/200-1/1,000. |
Reactivities | Mouse |
Host | Goat |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Native Mouse IgG1 heavy chain Protein. |
Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal. |
Formulation | 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 Label: AP State: Aff - Purified State: Liquid (sterile filtered) purified IgG fraction Stabilizer: 10 mg/ml BSA (Immunoglobulin and Protease free) Preservative: 0.09% (w/v) Sodium Azide Label: Alkaline Phosphatase (Calf Intestine) |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity Chromatography using Mouse IgG1 coupled to Agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
Conjugation | AP |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
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