Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) Goat Polyclonal Antibody

CAT#: AP33463HR-N

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, HRP, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Mouse IgG1 Peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
  Recommended Dilutions:
  ELISA: 1/20,000-1/100,000.
  Western Blot: 1/2,000-1/20,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Reactivities: Mouse
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Native Mouse IgG1 heavy chain Protein.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins.
  Specificity was confirmed by ELISA at less than 1% of target signal.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
  Stabilizer:
  10 mg/ml BSA (Immunoglobulin and Protease free)
  
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Peroxidase Conjugated
• Reconstitution Method: Restore with 1.0 ml deionized water (or equivalent)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Mouse IgG1 coupled to Agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.