Monkey IgA + IgG + IgM Goat Polyclonal Antibody

CAT#: R1250B

Monkey IgA + IgG + IgM goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/50,000-1/100,000.
  Western Blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/200-1/1,000.
• Reactivities: Monkey
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Monkey IgG, IgA and IgM whole molecule.
• Specificity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum.
  This product is suitable for the detection of all Monkey immuno-globulin classes, isotypes and chain combinations.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after restoration and standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below.
  Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.