Monkey IgA + IgG + IgM Goat Polyclonal Antibody

CAT#: R1250HRP

Monkey IgA + IgG + IgM goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/100,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/500-1/2,500.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Monkey IgG IgA and IgM whole molecules in goat.
• Specificity: This product was prepared from polyspecific antiserum by Immunoaffinity Chromatography using antigens coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgG, Monkey IgA and Monkey IgM.
  This reagent is suitable for the detection of all Monkey immunoglobulin subclasses and chain combinations.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free)
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Preservative: 0.01% (w/v) Thimerosal
  Label: Horseradish Peroxidase
  Conjugation Chemistry: Periodate
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• Purification: Immunoaffinity Chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.