Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1253AP

Mouse IgG (H+L chain) rabbit polyclonal antibody, AP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/2,000-1/10,000.
  Western blot: 1/500-1/2,500.
  Immunohistochemistry: 1/200-1/1,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, containing 10 mg/ml BSA (Ig and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: AP
  State: Liquid purified Ig fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV Absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  Dilute only prior to immediate use.
  Freezing Alkaline Phosphatase conjugates will result in a substantial loss of enzymatic activity.