Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1253B

Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/300,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4
  Label: Biotin
  State: Lyohilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
• Reconstitution Method: Reconstitute with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Storage: Store secondary antibody at 4° C prior to reconstitution.
  Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for several weeks at 4° C as an undiluted liquid.
  Dilute only prior to immediate use. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing!